Histone H4 full length human, recombinant, expressed in E. coli, >=80% (SDS-PAGE)

Histone H4 full length human, recombinant, expressed in E. coli, >=80% (SDS-PAGE)

Stock Code: 3592605
Manufacturer Part No: SRP0178-1MG
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Application

Suitable substrate for histone methyltransferases.

Biochem/physiol Actions

Histones H2B and H4 are found to become mobilized during an infection with HSV-1 (herpes simplex virus-1) and become available for binding to viral genome.

General description

Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), amino acids 2-104 (end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

ManufacturerSIGMA-ALDRICH
Formaqueous solution
Assay≥80% (SDS-PAGE)

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