Human Src has been used to study the kinetics of Src entrapped in a series of different sol-gel derived materials.
Biochem/physiol Actions
SRC proto-oncogene, non-receptor tyrosine kinase (SRC) phosphorylates membrane proteins and nucleoproteins and activates various intracellular signaling cascades. It has been associated with a number of human malignancies and cancers.
General description
SRC proto-oncogene, non-receptor tyrosine kinase (SRC) belongs to the family of non-receptor tyrosine kinases. It contains an amino-terminal domain, conserved Src-homology domains (SH2 and SH3), a tyrosine kinase domain and the carboxyl-terminus. The gene encoding this protein is localized on human chromosome 20q11.23.
Physical form
Solution in 50?mM Tris pH?7.5, 0.05?mM EDTA, 1?mM DTT, 100?mM NaCl, 0.05% NP-40, and 50% glycerol.
Unit Definition
One unit will phosphorylate one nmole of poly(Gly:Tyr) 4:1 per minute at pH 7.5 at 30°C.