Protein Kinase Cnu (PKCnu) Active human, recombinant, expressed in E. coli, >=75% (SDS-PAGE), buffer

Stock Code: 3592024
Manufacturer Part No: P7123-5UG

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Descriptions

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide (KRREILSRRPSYR) per minute per mg protein at 30 °C using a final concentration of 50 µM [³²P] ATP.

Biochem/physiol Actions

PKCΝ, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCΝ is an important physiologic target of the B-cell receptor (BCR) and exhibits activation upon BCR engagement.

Physical form

Solution of 5 µg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol