Calmodulin from bovine heart, lyophilized powder, 2,500-10,000 units/mg protein

Calmodulin from bovine heart, lyophilized powder, 2,500-10,000 units/mg protein

Stock Code: 3591934
Manufacturer Part No: P0270-200UN
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Application

This is the protein activator required for the Ca2+ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Biochem/physiol Actions

Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca2+ homeostasis.

Physical form

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Preparation Note

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Unit Definition

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30?°C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca2+.

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)calcium flux assay: suitable
CompositionProtein, ≥85% Lowry
Storage Temp.−20°C
Formlyophilized powder

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