alpha-Lactalbumin from bovine milk, Marker for non-denaturing PAGE

Stock Code: 3591636
Manufacturer Part No: L4385-1VL

Order Now for 7 day delivery

Click Add to Quote for price and delivery

Quantity: - +

Descriptions

Application

?-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.

Biochem/physiol Actions

?-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. ?-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native ?-lactalbumin from the reduced, denatured form. ?-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 ?° resolution, demonstrating four ?-helices and a triple stranded antiparallel ?-sheet.

Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp⁸⁷ or Asp⁸⁸ to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to ﹤3.5% of the maximal rate.

General description

?-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water