Pyrophosphatase, Inorganic from Escherichia coli, recombinant, expressed in E. coli, lyophilized pow

Stock Code: 3591549
Manufacturer Part No: I5907-1MG

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Descriptions

Application

Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.

Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i. It plays an important role in protein, RNA, and DNA synthesis.

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

General description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.

Other Notes

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg²⁺ dependent. It is a relatively thermostable protein.

Packaging

1 mg in glass bottle

Physical form

Lyophilized powder in Tris-buffered salts containing protease inhibitors

Unit Definition

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25?°C.