Coelenterazine, native, solid

Coelenterazine, native, solid

Stock Code: 3589819
Manufacturer Part No: C2230-50UG
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Application

Coelenterazine, native was used:-For Aequorin assaysFor measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cellsIn Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity

Biochem/physiol Actions

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca2+ binds to the complex; used to measure Ca2+ concentration in cells with high sensitivity and large dynamic range.

Caution

Protect from exposure to light

General description

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla,Oplophorus andPeriphylla.

Quality Level200
InChI keyYHIPILPTUVMWQT-UHFFFAOYSA-N
InChI1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitymethanol and ethanol: soluble
Formsolid
Assay≥95% (TLC)

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