Tris(2-carboxyethyl)phosphine hydrochloride solution, 0.5 M, pH 7.0(aqueous solution; pH was adjuste

Tris(2-carboxyethyl)phosphine hydrochloride solution, 0.5 M, pH 7.0(aqueous solution; pH was adjuste

Stock Code: 3758635
Manufacturer Part No: 646547-10X1ML
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Application


Tris (2-carboxyethyl) phosphine (TCEP) has also been used:to cleave cysteine residues in a synthetic peptidein reduction buffer for RNA Sequential Probing of Targets (SPOTs) imagingfor the reduction of oligonucleotidesas reducing agent during mitochondrial isolation


Tris (2-carboxyethyl) phosphine (TCEP) can be used in several downstream applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. It prevents oxidation of protein samples, which makes it a useful buffer component as it helps to preserve enzymatic activity. It has been used in the reduction and measurement of glutathione.


Biochem/physiol Actions


As a non-mercaptan reducing agent, it avoids the toxicity inherent in thiol-containing compounds. It is capable of disrupting the botulinum neurotoxin B heavy-chain/light-chain complex that is held together by a single disulfide bond, and that is responsible for endocytosis, and ultimately the toxicity, of the toxin. Since disulfide-coupled subunits are characteristic of many toxins (e.g., ricin, snake venom, and all BoNT serotypes), it may be useful as a rescue prophylactic in cases of toxin administration.


Tris(2-carboxyethyl)phosphine hydrochloride solution reduces the disulfide bonds and leaves other functional groups intact in proteins.


General description


Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution. It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents.


It belongs to the trialkylphosphine class.


Packaging


10×1 mL in ampule

Quality Level100
pH7.0(aqueous solution; pH was adjusted with ammonium hydroxide)
InChI keyPBVAJRFEEOIAGW-UHFFFAOYSA-N
InChI1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
ManufacturerSUPELCO
Density1.041 g/mL at 25 °C

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