Total Protein Kit, Micro,

Total Protein Kit, Micro,

Stock Code: 3589150
Manufacturer Part No: TP0100-1KT
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Application


Total protein kit, micro has been used to determine:total protein in the bacterial homogenate by Bradford methodtotal protein content by the Bradford microassay from the tissue extractsserum total proteinsoluble protein content by the Bradford method from leaves


Biochem/physiol Actions


Total protein kit, micro can be used to determine the concentration of proteins in solution.


General description


Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 µL or less sample.


Principle


Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.


Suitability


Suitable for protein determination for samples of up to 0.5 mg/ml.


Testing Method


Colorimetric, Endpoint

Quality Level100
ManufacturerSIGMA-ALDRICH
Parameter50 µL sample volume, 18-26 °C temperature
Further Infowavelength 595 nmtotal test time 2 minworking reagent stability 18-26°C for 2 weeksdynamic range 50 mg/dL 0.5 g/L
Storage Temp.2-8°C

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