Antibody is suitable for ELISA and immunoblotting.
Application
Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.
General description
The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.
The T7 tag is a 11 amino acid peptide, encoded in the leader sequence of T7 bacteriophage gene 10. This gene encodes a T7 major capsid protein.
Immunogen
synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH
Physical form
Solution at approximately 2?mg/ml in 0.01?M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.