QuantiPro BCA Assay Kit, for 0.5-30 mug/ml protein

Stock Code: 3587308
Manufacturer Part No: QPBCA-1KT
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Application


In addition to protein determination and quantification in solution, the QuantiPro BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.


The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5?to 30?µg/mL in tube assays and 1?to 20?µg/mL in 96- or 384-well plate assays.


Features and Benefits


Accurate across a broad range of protein concentrations High sensitivity; linear response form 0.5 to 30 µg/ml of protein Stable color complex Reduced susceptibility to detergents


Legal Information


QuantiPro is a trademark of Sigma-Aldrich Co. LLC


Principle


Based on the alkaline reduction of Cu2+ to Cu1+ by proteins, and the formation of a bicinchoninic acid:Cu1+ complex having an absorbance maximum at 562 nm.


Storage and Stability


Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C

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