Amino-terminal FLAG-BAP Fusion Protein,

Stock Code: 3587282
Manufacturer Part No: P7582-100UG
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Application


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Amino-terminal FLAG-BAP Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.


General description


Amino-terminal FLAG-BAP Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45?55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.


The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.


Legal Information


FLAG is a registered trademark of Sigma-Aldrich Co. LLC


FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC


Other Notes


Control protein


Physical form


Protein is supplied in 10 mM Tris, 120 mM NaCl,0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formliquid

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