Carboxy-terminal FLAG-BAP Fusion Protein,

Stock Code: 3587281
Manufacturer Part No: P7457-.1MG
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Application


Carboxy-terminal FLAG-BAP Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).


Biochem/physiol Actions


The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.


General description


Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).


Legal Information


FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC


Other Notes


Control protein


Physical form


Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2


Preparation Note


Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formliquid

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