Carboxy-terminal FLAG-BAP Fusion Protein,

Stock Code: 3587281
Manufacturer Part No: P7457-.1MG

Order Now for 21 day delivery

Click Add to Quote for price and delivery

Quantity: - +

Descriptions

Application

Carboxy-terminal FLAG-BAP^™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).

Biochem/physiol Actions

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

General description

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Legal Information

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Control protein

Physical form

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Preparation Note

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml