Lectin from Erythrina cristagalli (coral tree), lyophilized powder

Stock Code: 3587080
Manufacturer Part No: L5390-5MG

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Descriptions

Analysis Note

Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH?6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25?°C.

Biochem/physiol Actions

Lectin or haemagglutinin is a sugar stereospecific binding protein or glycoprotein. It is capable of reversibly agglutinating cells. It is implicated in various cellular processes such as cell agglutination, homing of leukocytes, induction of mitosis in lymphocytes, interferon c (IFN c) and cytotoxicity. It is used as a blood typing reagent in immunohaematology. Lectin is also used as a reagent in testing erythrocyte polyagglutinable states. It is considered as a potential tool for analyzing cell surface architecture and related membrane functions.

ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.

Packaging

5 mg in glass bottle

10 mg in poly bottle

Preparation Note

Purified by affinity chromatography.