Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (cont

Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (cont

Stock Code: 3587043
Manufacturer Part No: L1668-5MG
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Analysis Note


Potency and solubility expressed in mg lectin/ml


Application


Lectin from Phaseolus vulgaris (red kidney bean) has been used:for the stimulation of cluster of differentiation (CD4+) T cellsfor the activation of primary peripheral blood lymphocytesfor the stimulation of peripheral blood mononuclear cells


Biochem/physiol Actions


PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity. PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.


Packaging


Sold on the basis of lectin content

Colourwhite to off-white
Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.2-8°C
SolubilityPBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)
Formlyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

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