Lectin from Pisum sativum (pea), FITC conjugate, lyophilized powder

Stock Code: 3587026
Manufacturer Part No: L0770-2MG
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Analysis Note


Agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.


Application


Lectin from Pisum sativum (pea) has been used to determine acrosomal status and viability of spermatozoa and to assess sperm acrosome reaction (AR) patterns


Biochem/physiol Actions


PSA is not blood group specific, but has an affinity for terminal α-D-mannosyl residues. PSA is a mitogen similar to concanavalin A.


Lectin from Pisum sativum (pea) possess anti-hepatoma effect and mitogenicity to lymphocytes, like concanavalin A (Con A).


General description


Lectin from Pisum sativum (pea) is a tetrameric glycoprotein of about 48 kDa. It is isolated from peas. Pisum sativum agglutinin is specific for ?-D-glucose and ?-D-mannose.


Packaging


2 mg in serum bottle


Preparation Note


Conjugates are prepared from affinity purified lectin.

Quality Level200
ManufacturerSIGMA-ALDRICH
CompositionProtein, ≥95% biuret
Storage Temp.−20°C
Impuritiessalt, free
Formlyophilized powder

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