Invertase Glycoprotein Standard/280), BioReagent, from Saccharomyces cerevisiae, for proteomics

Stock Code: 3587005
Manufacturer Part No: I0408-500UG

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Descriptions

Application

Used in the production of confectionary foods and artificial honey.

Invertase Glycoprotein Standard has been used:to generate N-linked glycan libraryas a negative control to study the binding of lectins to high mannose structuresfor sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Other Notes

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.