beta-Glucuronidase Reporter Gene Staining Kit,

beta-Glucuronidase Reporter Gene Staining Kit,

Stock Code: 3586998
Manufacturer Part No: GUSS-1KT
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Application


The E. coli GUS gene is extensively used to analyze gene expression in transformed plants. Plants have low intrinsic GUS activity, and the E. coli gene is quite stable in plant cells. As a tag, GUS remains active at the N-terminus of fusion proteins.Kit is suitable for biochemical activity assays, immunological assays and histochemical staining of tissue and cells.


Features and Benefits


Histochemical staining of plant tissues expressing the E. coli GUS enzyme Ideally suited to plant expression studies due to very low GUS activity in plants and high enzyme stability GUS does not interfere with plant cell function or viability


General description


Kit is used to anaylze β glucuronidase (E.coli GUS gene) expression in transformed plants.


Other Notes


The substrate used in this kit is X-GlcA (5-bromo-4-chloro-3-indolyl β-D-glucuronide) which results in an insoluble indigo-blue precipitate in transfected cells and tissues.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C

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