INTEGRIN MED ADHSN ARRYS ALPHA

INTEGRIN MED ADHSN ARRYS ALPHA

Stock Code: 3586886
Manufacturer Part No: ECM530
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Application


The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface. This assay can substitute for FACS analysis (3) and is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.



For Research Use Only. Not for use in diagnostic procedures.


Research CategoryCell Structure


This assay can substitute for FACS analysis & is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.


Components


Alpha Integrin Array Plate: (PN: 90599) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-alpha integrin monoclonal antibodies: (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, alphavbeta3), and one goat anti-mouse negative well. See the plate layout on product insert.Cell Stain Solution: (Part No. 90144) One 20 mL bottle.Extraction Buffer: (Part No. 90145) One 20 mL bottle.Assay Buffer: (Part No. 90601) One 100 mL bottle.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Introduction

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. A family of cell surface receptors, the integrins, mediates cell attachment to proteins of the extracellular matrix. Integrins are heterodimer molecules containing an alpha and beta transmembrane glycoprotein subunit that are non-covalently bound together (1-2). Historically, antibodies have been used to determine the integrin profiles of cells by immunoprecipitation, immunofluorescence, immunoblotting, and flow cytometry. These methods are time-consuming, laborious or require sophisticated equipment.

The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of alpha integrin surface expression.

In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

⋅ Beta Integrin-Mediated Cell Adhesion Array (ECM531)

⋅ Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (ECM532)

⋅ QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)

⋅ QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)

⋅ QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell based assay: suitable

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