GenElute#-E Single Spin Cap Puncher

GenElute#-E Single Spin Cap Puncher

Stock Code: 3586876
Manufacturer Part No: EC9999-1EA
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Application


The GenElute™-E Cap Puncher is designed to work with the GenElute™-E line of DNA and RNA purification kits. When pressed onto the lid of the single-spin column, the cap puncher creates a small opening through which a sample can be pipetted directly onto the column. This reduces the amount of handling by eliminating the need for cap removal, and lessens the chance of cross contamination when handling multiple samples.For full instructions, please reference any of the GenElute™-E protocols.


General description


We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.


GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers: Isolation of DNA in under an hour with minimal sample handling Better purity, leading to improved performance in PCR and other applications Significant reduction in plastic and hazardous chemical wasteTraditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.GenElute™-E kits employ size exclusion?negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and?plastic?materials required for purification.?This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.


Legal Information


SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany


GenElute is a trademark of Sigma-Aldrich Co. LLC


Other Notes


We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. ?GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste. GenElute™

ManufacturerSIGMA-ALDRICH
FeatureIntended use (For use with GenElute™-E Single Spin purification columns)

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