Endoglycosidase F3 from Elizabethkingia miricola, recombinant, expressed in E. coli, 30 U/mg

Endoglycosidase F3 from Elizabethkingia miricola, recombinant, expressed in E. coli, 30 U/mg

Stock Code: 3586833
Manufacturer Part No: E2264-.2UN
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Application


Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.


Biochem/physiol Actions


Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates ?-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.


Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.


Packaging


Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH?4.5


Physical form


Aseptically filled solution in 20 mM Tris-HCl, pH 7.5


Unit Definition


One unit will release N-linked oligosaccharides from 1 µmole of denatured porcine fibrinogen in 1 minute at 37?°C, pH 4.5.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formsolution

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