Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

Cytochrome c Oxidase Assay Kit, sufficient for 100 tests, soluble and membrane bound mitochondria

Stock Code: 3586746
Manufacturer Part No: CYTOCOX1-1KT
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Application


The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.


Biochem/physiol Actions


Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.


Features and Benefits


Simple, optimized protocol - Obtain reproducible results without special training needs Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility Use to analyze the intactness of mitochondrial membranes Simple colorimetric measurement of solution

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C

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