CelLytic PN Isolation/Extraction Kit, For plant leaves

Stock Code: 3586722
Manufacturer Part No: CELLYTPN1-1KT
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Application


The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.


General description


CelLytic-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.


Legal Information


CelLytic is a trademark of Sigma-Aldrich Co. LLC


Other Notes


This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C

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