Concanavalin A from Canavalia ensiformis (Jack bean), Type IV-S, lyophilized powder, gamma-irradiate

Concanavalin A from Canavalia ensiformis (Jack bean), Type IV-S, lyophilized powder, gamma-irradiate

Stock Code: 3586661
Manufacturer Part No: C0412-5MG
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Analysis Note


Where reported, agglutination activity is expressed in µg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.


Application


Concanavalin A from Canavalia ensiformis (jack bean) has been used:to induce spleen blastogenesisto generate ConA blast targets from B6 and lpr spleen cellsto stimulate splenic lymphocytes from estrogen-and placebo-treated mice


Biochem/physiol Actions


Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.−20°C
Impuritiessalt, essentially free
SolubilityPBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy
Formlyophilized powder

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