N-Acetylneuraminic Acid Aldolase from Escherichia coli, lyophilized powder, >=20 units/mg protein (b

Stock Code: 3586572
Manufacturer Part No: A6680-25UN
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Application


This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid whencoupled with the related enzymes in clinical analysis.For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.


Used in the Sialic Acid Quantification Kit, SIALIC-Q


Packaging


25 units in glass bottle


Physical form


Lyophilized powder containing mannitol and EDTA


Physical properties


Isoelectric point: 4.6 ± 0.1Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)Structure: 3 subunits (approx. 35,000) per mol of enzymeInhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+ Optimum pH: 7.5– 8.0Optimum temp: 70°CpH Stability: pH 6.0–9.0 (10°C, 25hr)Thermal stability: Below 65°C (pH 7.5, 30 min)


Unit Definition


One unit will release 1.0 µmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formlyophilized powder

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