Monoclonal Anti-polyHistidine-Agarose antibody produced in mouse, clone HIS-1, purified immunoglobul

Monoclonal Anti-polyHistidine-Agarose antibody produced in mouse, clone HIS-1, purified immunoglobul

Stock Code: 3586567
Manufacturer Part No: A5713-1ML
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Application


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)


Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors


General description


This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.


Immunogen


recombinant polyHistidine tagged fusion protein.


Legal Information


This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.


Physical form


Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Preparation Note


Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.


Specificity


The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Capacity15-30 nmol/mL binding capacity
ManufacturerSIGMA-ALDRICH
FormPBS suspension

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