Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody produced in mouse, clone HIS-1, purified

Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody produced in mouse, clone HIS-1, purified

Stock Code: 3586566
Manufacturer Part No: A5588-.5ML
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Application


Antibody is suitable for ELISA, immunoblotting and dot blotting assays.


General description


The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.


Immunogen


recombinant polyHistidine tagged fusion protein.


Legal Information


This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.


Physical form


Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide


Specificity


The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Quality Level200
ManufacturerSIGMA-ALDRICH
Formbuffered aqueous glycerol solution

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