ChIPAb+ Phospho-Histone H3 (Ser10);

Stock Code: 3586475
Manufacturer Part No: 17-685

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Descriptions

Analysis Note

ControlIncludes negative control mouse IgG antibody and control primers specific for human GAPDH promoter.

Application

Research CategoryEpigenetics & Nuclear Function

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated or colcemid-treated HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-phospho-Histone H3 (Ser10) antibody and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of phospho-histone H3 (Ser10)-associated DNA fragments was verified by qPCR using Control Primers for untreated and treated chromatin samples (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Western Blot Analysis:
Recombinant Histone H3 or acid extracts from colcemid-treated HeLa cells (1 µg) were resolved by electrophoresis, transferred to PVDF membrane and probed with 1 µg/mL anti-phospho Histone H3 (Ser10), clone CMA312. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

This ChIPAb+ Phospho-Histone H3 (Ser10) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

Research Sub CategoryChromatin BiologyHistones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Phospho-Histone H3 (Ser10) set includes the Anti-phospho-Histone H3 (Ser10) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 166 bp region within the promoter of the human GAPDH gene. The phospho-histone H3 (Ser10) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of phospho-histone H3 (Ser10) associated chromatin.

Immunogen

Epitope: a.a. 1-19

Immunogen was a synthetic peptide corresponding to amino acids 1-19 of histone H3, phosphorylated at Ser10.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany