ChIPAb+ Acetyl Histone H4

ChIPAb+ Acetyl Histone H4

Stock Code: 3586455
Manufacturer Part No: 17-630
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Analysis Note


ControlIncluded negative control rabbit IgG antiserum and control primers specific for human GAPDH promoter.


Application


Research Sub CategoryHistones


This ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.


Research CategoryEpigenetics & Nuclear Function


Western Blot Analysis:
Acidextracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl Histone H4 (1:2000).
Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemi-luminescence detection system. (Please see figures).


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.


Immunogen


The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.


Legal Information


UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Packaging


25 assays per set. ~4 µL per chromatin immunoprecipitation


Physical form


Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100 µL of antiserum containing 0.05% sodium azide. Store at -20°C.

Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% sodium azide. Store at -20°C.

Control Primers. One vial containing 75 µL of 5 µM of each primer specific for for human GAPDH.


Quality


Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 µL of either a normal rabbit antiserum or Anti-Acetyl Histone
H4 serum and the Magna ChIP A (Cat. # 17-610) Kit

Quality Level100
ManufacturerSIGMA-ALDRICH

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