ChIPAb + CREB

ChIPAb + CREB

Stock Code: 3586441
Manufacturer Part No: 17-600
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£711.75 (exc VAT) per EACH
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Analysis Note


ControlIncludes negative control cultured supernatant and primers specific for of human cFos CRE.


Application


Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from 293T cells (5 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 10 µL of either a negative supernatant, or CREB antibody and the Magna ChIP® A Kit (Cat. # 17-610).
Successful immunoprecipitation of CREB-associated DNA fragments was verified by qPCR using cFos downstream 4Kb (negative) and cFos CRE (positive) (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB, clone NL904 (1:8,000 dilution)
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates CREB (~43 kDa).


Research CategoryEpigenetics & Nuclear Function


This ChIPAb+ CREB -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.


Research Sub CategoryChromatin Biology


Components


Anti-CREB (Rabbit monoclonal IgG): Part No. CS203204. One vial containing 250 µL of cultured supernatant containing 0.05% sodium azide. Store at -20°C.
Negative Control Supernatant: Part No. CS203202. One vial containing 250 µL of cultured supernatant containing 0.05% sodium azide. Store at -20°C.
ChIP Primers cFos CRE: Part No. CS203203. One vial containing 75 µL of 5 µM of each primer specific for the cFos CRE. Store at -20°C.
FOR: GGC CCA CGA GAC CTC TGA GAC A
REV: GCC TTG GCG CGT GTC CTA ATC T


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ CREB, antibody/primer set includes the CREB, antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 64 bp region flanking the CRE of the human cFos gene

Quality Level100
ManufacturerSIGMA-ALDRICH

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