Magna ChIP G Tissue Kit

Magna ChIP G Tissue Kit

Stock Code: 3586411
Manufacturer Part No: 17-20000
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Application


The Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable & site specific tissue biopsies.


Components


Magnetic Protein G beads ChIP Dilution Buffer Low Salt Wash Buffer High Salt Wash Buffer LiCl Wash Buffer TE Buffer Tissue Lysis Buffer 10X Tissue Stabililizing Solution ChIP Elution buffer (w/o Proteinase K) 10X Glycine 10X PBS 1mm Micro-dissection punch Spin Filters Collection Tubes Bind Reagent A Wash Reagent B Elution Reagent C Protease Inhibitor Cocktail II Proteinase K


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair. Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.


Legal Information


MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany


Packaging


22 ChIP assays from tissue samples.Two modules containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions from tissue samples.


Storage and Stability


Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.

Quality Level100
ManufacturerSIGMA-ALDRICH

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