ChIPAb+ Androgen Receptor

Stock Code: 3586393
Manufacturer Part No: 17-10489

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Descriptions

Analysis Note

ControlIncludes normal rabbit IgG and primers specific for prostate-specific antigen (PSA) enhancer region.

Application

Research Sub CategoryTranscription Factors

Chromatin Immunoprecipitation:
LNCaP cells were hormone starved for 72 hours, then induced overnight with 1 µM CI-4AS-1. ChIP was performed using ~1e5 cell equivalents per IP using 1 µg of either Normal Rabbit IgG (Part No. PP64B), or 1 µg Anti-Androgen Receptor (Part No.CS207339) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of androgen receptor associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) as a positive locus, and GAPDH Promoter (Part No. 22-004) as a negative locus. Data are presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

Chromatin Immunoprecipitation:
LNCaP cells were hormone starved for 72 hours, then induced overnight with vehicle (untreated) or 1 µM CI-4AS-1 (treated). ChIP was performed using ~ 1e5 cell equivalents per IP using 3 µg of either Normal Rabbit IgG (Part No.PP64B ), or 3 µg Anti-Androgen Receptor (Part No.CS207339) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of androgen receptor associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) and the negative locus was the HNRP2AB1 primer set. Delta delta Ct calculations reporting fold enrichment at the positive locus over the negative locus are reported.
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

Western Blot Analysis:
A 1:200-1:500 dilution of this antibody detected Androgen Receptor in RIPA lysates from 20 µg of human LNCaP cells.

Immunoprecipitation:
A representative lot immunoprecipitated Androgen Receptor from 500 µg of LNCaP RIPA lysate.

Immunocytochemistry:
A 1:50 dilution of a representative lot showed positive immunostaining for Androgen Receptor in LNCaP cells fixed with 2% formaldehyde/5% acetic acid and permeabilized with 0.1% Triton X-100.

Research CategoryEpigenetics & Nuclear Function

ChIPAb+ &rogen Receptor set includes the &rogen Receptor antibody, a Normal Rabbit IgG, & control primers, which amplify a 85 bp region of the human prostate specific antigen (PSA) enhancer region.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time