ChIPAb+ CBX8

ChIPAb+ CBX8

Stock Code: 3586333
Manufacturer Part No: 17-10135
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Analysis Note


ControlIncludes normal rabbit IgG and primers specific for the human HOXA2 promoter region.


Application


Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min (1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-CBX8 (Part No. CS207348) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, HoxA2 upstream (Part No. CS203200) as a positive locus, and GAPDH promoter primers (Part No. 22-004) as a negative locus. Data are presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera-2 cells fixed for 15 min ( 1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-CBX8 (Part No. CS207348) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of CBX8 associated DNA fragments was verified by qPCR using ChIP Primers, Hox A2 upstream (Part No. CS203200).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected CBX8 on 5, 15 and 50 µg of HeLa whole cell lysate and 50 µg of 293T whole cell lysate.


Research CategoryEpigenetics & Nuclear Function


Research Sub CategoryChromatin Biology


Use ChIPAb+ CBX8 ChIP Validated rabbit polyclonal antibody for the detection of CBX8 also known as Chromobox protein homolog 8 & Polycomb 3 homolog.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


The family of Heterochromatin Proteins ("Chromobox Homolog", CBX) are highly conserved adapter molecules, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.


All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)western blot: suitable, ChIP: suitable

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