ChIPAb+ TCF-4; clone 6H5-3

ChIPAb+ TCF-4; clone 6H5-3

Stock Code: 3586326
Manufacturer Part No: 17-10109
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Analysis Note


ControlIncludes negative control normal mouse IgG and primers specific for human SP5 promoter.


Application


Research Sub CategoryTranscription Factors


Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HT29 cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of Normal Mouse IgG or 2 µg Anti-TCF-4 and the Magna ChIP® G Kit (Cat. # 17-611).
Successful immunoprecipitation of TCF-4 associated DNA fragments was verified by qPCR using ChIP Primers, SP5 Promotor as a positive locus, and GAPDH promoter primers as a negative locus (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Western Blot Analysis:
Representative lot data.
Cos-1 cells were transfected with TCF-4 cDNA in pUSE (lane 2) or pUSE (lane 1). RIPA lysates were prepared, resolved by electrophoresis, transferred to nitrocellulose and probed with anti-TCF-4 (1 mg/mL).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates TCF-4 (~66 kDa) (Please see figures).

Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).

Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with Beta-Catenin (Persad, S., 2001).

Supershift Assay (EMSA):
A previous lot of this antibody was reported to supershift TCF-4/DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).


Research CategoryEpigenetics & Nuclear Function


This ChIPAb+ TCF-4 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.


All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context

Quality Level100
ManufacturerSIGMA-ALDRICH

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