EZ-Magna ChIP A/G

Stock Code: 3586321
Manufacturer Part No: 17-10086

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Descriptions

Application

Research CategoryEpigenetics & Nuclear Function

Used to detect/quantify: Protein A+G

Components

Magnetic Protein A/G BeadsChIP Dilution BufferLow Salt Wash BufferHigh Salt Wash BufferLiCl Wash BufferTE BufferCell Lysis BufferNuclear Lysis BufferChIP Elution Buffer (w/o Proteinase K)Proteinase KRNase A10X Glycine10X PBSProtease Inhibitor Cocktail IIAnti-RNA polymerase II, clone CTD4H8Normal Mouse IgGControl PrimersSpin FiltersCollection TubesBind Reagent AWash Reagent BElution Reagent C

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Unlike standard ChIP protocols that can be laborious and time consuming, the Magna ChIP kit protocol can reduce the amount of time required to perform a ChIP experiment from three days to one. Additionally, the smaller Magna ChIP reaction volume increases the relative concentration of the antibody enabling the ChIP reaction to be performed with reduced amounts of both antibody and sheared chromatin. Finally because this kit uses a blend of protein A and protein G beads, a wider range of antibody isotypes can be used than A or G alone. This allows a wider variety of antibodies to be used and avoids the need to purchase separate kits for protein A and protein G based immunoprecipitation. Because Magna ChIP kits use paramagnetic beads they are compatible with automated high throughput platforms, thus allowing a large number of ChIP reactions to be carried out simultaneously. Features & Benefits: Magnetic bead-based rapid protocol allows performance of ChIP in as little as 1 day Blend of protein A+G bead blend allows ChIP using a broader range of antibodies than A or G alone Negative and positive control antibodies and control primer set to simplify validation of experimental procedure Includes spin columns to make DNA purification easier and more reliable - no more messy phenol-chloroform extractions Complete kit with all required reagents for reliable and reproducible results Compatible with ChIPAb+ validated antibody and primer sets Chromatin Immunoprecipitation (ChIP) is an important technique allowing the analysis of in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc