ChIPAb+ Acetyl-Histone H4 (Lys5)

ChIPAb+ Acetyl-Histone H4 (Lys5)

Stock Code: 3586309
Manufacturer Part No: 17-10045
Order Now for 7 day delivery
£654.64 (exc VAT) per EACH
Quantity: - +

Analysis Note


ControlIncludes negative control supernatant and primers specific for human GAPDH.


Application


Research Sub CategoryHistones


Research CategoryEpigenetics & Nuclear Function


This ChIPAb+ Acetyl-Histone H4 (Lys5) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.


Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either a negative control supernatant , or 2 µL of Anti-Acetyl-Histone H4 (Lys5) and the Magna ChIP® A Kit (Cat. # 17-610).
Successful immunoprecipitation of acetyl-Histone H4 (Lys5) associated DNA fragments was verified by qPCR using control Primers flanking the human GAPDH promoter, or primers amplifying the promoter of human β-globin, which is transcriptionally inactive in HeLa cells (Please see figures).
Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.

Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were probed with anti-acetyl-Histone H4 (Lys5) (1:1,000 dilution).
Proteins were visualized using goat anti-rabbit. (Please see figures).

Western Blot Analysis/Peptide Inhibition: Representative lot data.
Sodium butyrate treated HeLa acid extract was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl-Histone H4 (Lys5) (1:1000 dilution).
To demonstrate specificity, serum was preincubated with modified Histone H4 peptides:

Lane 1: No peptide
Lane 2: Acetyl-Histone H4 (K5) (Cat. # 12-343)
Lane 3: Acetyl-Histone H4 (K8) (Cat. # 12-344)
Lane 4: Acetyl-Histone H4 (K12) (Cat. # 12-345)
Lane 5: Acetyl-Histone H4 (K16) (Cat. # 12-346)
Lane 6: Acetyl-Histone H4 (K5, 8, 12, 16) (Cat. # 12-353)
Lane 7: Histone H4 (Cat. # 12-347)

Proteins were visualized using a donkey anti-rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system. (Please see figures).


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).


Quality Level100
ManufacturerSIGMA-ALDRICH

There are no downloads for this product.