RIPAb+ CUGBP2; clone IH2

RIPAb+ CUGBP2; clone IH2

Stock Code: 3586257
Manufacturer Part No: 03-119
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Analysis Note


ControlIncludes negative control mouse IgG antibody and control primers specific for the cDNA of human JUN.


Application


Research Sub CategoryRNA Metabolism & Binding ProteinsRNA Binding Protein (RBP)


Research CategoryProtein TraffickingEpigenetics & Nuclear Function


This RIPAb+ CUGBP2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.


Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from Jurkat cells (~5 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. #CS200621), or 5 µg of Anti-CUGBP2 antibody (Cat. # CS204360).
Precipitated proteins (lane 1: mouse IgG, lane 2: anti-CUGBP2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CUGBP2 antibody (Cat. # CS204360, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231)
Arrow indicates CUGBP2 (Figure 2).
Western Blot Analysis:
Representative lot data.
Lysates from HL60 cells were probed with a previous lot of Anti-CUGBP2 (1:1000 dilution).
Arrow indicates CUGBP2 ~56 kDa (Figure 3).


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
CUGBP2 is a RNA-binding protein implicated in the regulation of several post-transcriptional events including pre-mRNA alternative splicing, mRNA translation and stability. CUGBP2 mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.


Immunogen


A synthetic peptide corresponding to amino acids 209-415 of human CuGBP2.


Legal Information


UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany


Other Notes


Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Packaging

Quality Level100
ManufacturerSIGMA-ALDRICH

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