Analysis Note
ControlIncludes negative control mouse IgG antibody and control primers specific for mouse Iqsec1.
Application
Western Blot Analysis:
Representative lot data.
Mouse E17 spinal cord lysate (Lane 1), Mouse E16 brain (Lane 2) and NIH/3T3 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Fragile X Mental Retardation Protein (1:500 dilution of a previous lot).
Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Fragile X Mental Retardation Protein (~71 kDa) (Please see figures).
Immunohistochemistry: Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA: A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears: A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry: A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only) (Please see figures).
Optimal working dilutions must be determined by the end user.
This RIPAb+ Fragile X Mental Retardation Protein -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Research CategoryEpigenetics & Nuclear FunctionNeuroscience
Research Sub CategoryRNA Binding Protein (RBP)RNA Metabolism & Binding ProteinsNeurodegenerative Diseases
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
Immunogen
Fusion protein with a full length FMRP (human).
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Packaging
10 assays per set