Sera-Mag Magnetic Olido(dt) particles, Cytiva, 38152103011150, 1 mum avg. part. size, suspension (1%

Stock Code: 2796431
Manufacturer Part No: GE38152103011150

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Descriptions

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Sera-Mag^™ SpeedBeads and Sera-Mag^™ Carboxylate-Modified Magnetic Particles typical application uses include sample preparation, proteomics, nucleic acid isolation, and immunoassay applications.

Sera-Mag^™ SpeedBeads and Sera-Mag^™ Carboxylate-Modified Magnetic Particles typical application uses include sample preparation, proteomics, nucleic acid isolation, and immunoassay applications.Sera-Mag^™ Magnetic Olido(dt) particles can be used for convenient isolation and extraction of mRNA from a variety of sources for downstream applications such as RT-PCR, cDNA library construction, cDNA microarrays, affinity purification, primer extension and subtractive hybridization.

Features and Benefits

Sera-Mag^™ SpeedBeads and Sera-Mag^™ Carboxylate-Modified Magnetic Particles allow convenient covalent coupling of target molecules.•Carboxylic groups on the surface permit easy covalent coupling to biomolecules of interest using convenient carbodiimide chemistry.•Different levels of hydrophobicity/hydrophilicity available.•Available in Sera-Mag^™ SpeedBeads and original Sera-Mag^™ versions.•Excellent sensitivity and low nonspecific binding for greater accuracy.•Fast Magnetic Response Times and Stable Physical Integrity.•Very high, specific poly A⁺ binding capacity ensures maximum extraction of mRNA.•Covalently bound oligo(dT)14 prevents leaching from the particle surface.•Encapsulation means no exposed iron and minimized interference with downstream enzymatic applications.•Uniform, nominal 1 μm diameter provides high surface area and excellent lot-to-lot reproducibility.

General description

Sera-Mag^™ SpeedBeads and Sera-Mag^™ Carboxylate-Modified Magnetic Particles combine a fast magnetic response time and high binding capacity with a large surface area, high sensitivity, stability, physical integrity, and fast reaction kinetics. Covalent coupling of biomolecules such as proteins and nucleic acids to carboxyl groups on the surface is easily accomplished using our standard coupling technology. The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One (Sera-Mag^™) or two (Sera-Mag^™ SpeedBeads) layers of magnetite are then coated onto this core, while the surface is modified to minimize non-specific binding of proteins.

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