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Application
His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD ﹤ 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.
Features and Benefits
Suitable for purification of multiple samples in parallel, such as screening. Convenient, ready-to-use single-use spin column, prepacked with 100 µL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media. With a microcentrifuge, one run takes about 10 minutes.
General description
His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.