Protein A High Performance Spintrap, Cytiva, 28-9031-32, pack of 16 columns

Stock Code: 2796344
Manufacturer Part No: GE28-9031-32
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Analysis Note


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Application


Protein A Sepharose® HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.


Features and Benefits


For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use. Classic and cross-link protocols provide flexibility. Elution conditions formatted for both electrophoresis and LC-MS analysis workflows. Fast binding kinetics and high capacity provide high yield. Prepacked with Protein A Sepharose® High Performance for coupling of antibodies of IgG subclasses. Easy scale-up with HiTrap® Protein A HP prepacked columns.


General description


protein A HP SpinTrap are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose® HP.


Legal Information


Sepharose is a registered trademark of Cytiva


SpinTrap is a trademark of Cytiva


HiTrap is a registered trademark of Cytiva

Capacity>1 mg binding capacity(human IgG well)
Matrixhighly cross-linked 6% agarose
Featurewetted part: no

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