GSTrap High Performance, Cytiva, 17-5282-01, pack of 1 x 5 mL

GSTrap High Performance, Cytiva, 17-5282-01, pack of 1 x 5 mL

Stock Code: 2796256
Manufacturer Part No: GE17-5282-01
Order Now for 21 day delivery
£476.36 (exc VAT) per EACH
Quantity: - +

Analysis Note


To view the Certificate of Analysis for this product, please visit www.cytiva.com.


Application


GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose® High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose® High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.


Features and Benefits


High resolution purification of GST-tagged Proteins. Mild elution conditions preserving Protein antigenicity and function. Fast, one-step purification. Easy to use with syringe, pump or a chromatography system.


General description


GSTrap™ HP columns are prepacked with Glutathione Sepharose® High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.


Legal Information


Sepharose is a registered trademark of Cytiva


GSTrap is a trademark of Cytiva


Storage and Stability


Store at 4 to 30 °C (20% Ethanol)

Capacity>7 mg/mL binding capacity (medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography
Matrixhighly cross-linked 6% agarose

There are no downloads for this product.