IMAC Sepharose High Performance,Cytiva, 17-0920-07, pack of 100 mL

IMAC Sepharose High Performance,Cytiva, 17-0920-07, pack of 100 mL

Stock Code: 2796198
Manufacturer Part No: GE17-0920-07
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Analysis Note


To view the Certificate of Analysis for this product, please visit www.cytiva.com.


Application


Conveniently charge with your metal of choice. IMAC Sepharose® FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.


Features and Benefits


For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion Conveniently charge with your metal of choice 34 µm bead size for high performance purification High binding capacity


General description


IMAC Sepharose® High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.


Legal Information


Sepharose is a registered trademark of Cytiva


IMAC Sepharose products, Ni Sepharose products and Fe Sepharose productsThese products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.


Storage and Stability


Store at 4 to 30 °C (20% Ethanol)

Capacity≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Matrixhighly cross-linked 6% agarose

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