REDExtract-N-Amp Seed PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

REDExtract-N-Amp Seed PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications

Stock Code: 3582527
Manufacturer Part No: XNASS-1KT
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£51.26 (exc VAT) per 1KIT
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Application


REDExtract-N-Amp™ seed PCR Kit has been used for DNA extraction


Features and Benefits


Perfect for quick genotyping results Rapid extraction of genomic DNA for PCR in 15 minutes No long enzymatic digestions Hot Start antibody for highly specific PCR amplification of genomic DNA Compatible with a wide variety of seed sources Extracts are stable at 4 °C for at least 6 months, allowing for multiple reassays Phenol/chloroform extraction or alcohol precipitation not required Column purification or centrifugation not required


General description


The REDExtract-N-Amp™ Seed PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of plant seeds and amplify targets of interest by direct PCR (polymerase chain reaction). A novel extraction method eliminates the need for long enzymatic digestions. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.


Legal Information


Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC


REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC


Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. SYBR Green nucleic acid stain is licensed from Molecular Probes, Inc. under US Patent No. 5,436,134 for use in Real-Time PCR by end-users for research and development only. SYBR is a registered trademark of Molecular Probes, Inc.Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.


Other Notes


For additional information, please see www.sigma-aldrich.com/extract-n-amp.


Principle


The kit comes with validated protocols to extract and amplify genomic DNA from corn, wheat, sunflower, cotton, soybean, sorghum, canola, and Arabidopsis seeds. Additional protocols are available. In a typical procedure, genomic DNA is extracted from a sample that has been ground and incubated in the seed preparation solution and extraction solution for 10 minutes at room temperature

Quality Level200
ManufacturerSIGMA-ALDRICH
Technique(s)PCR: suitable
Featurehotstart, dNTPs included
Storage Temp.−20°C

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