Quantitative RT-PCR ReadyMix, One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Quantitative RT-PCR ReadyMix, One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

Stock Code: 3582428
Manufacturer Part No: QR0200-1KT
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Application


Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).


Features and Benefits


The master mix allows consistency and reproducibility from one reaction to the next Reduced risk of contamination from multiple pipetting steps Reduced set-up time as compared to manual or wax Hot Start methods JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation Broad instrument compatibility Includes a separate ROX reference dye vial for reaction normalization


General description


Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.


Legal Information


This product is for research use only.


JumpStart is a trademark of Sigma-Aldrich Co. LLC


ReadyMix is a trademark of Sigma-Aldrich Co. LLC


Eppendorf is a registered trademark of Eppendorf AG


Packaging


1 kit sufficient for 250 reactions at 20 µL each or for 100 reactions at 50 µL each.

Colourcolorless
Quality Level300
ManufacturerSIGMA-ALDRICH
CompatibilityBio-Rad iQ5, Bio-Rad MiniOpticon, Strategene Mx3000P, Eppendorf® Mastercycler ep realplex, ABI ViiA 7, Qiagen Corbett Rotor-Gene Q, ABI 7500, Qiagen Corbet
Featurehotstart, dNTPs included
Storage Temp.−20°C

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