Inhibitor Resistant Genotyping PCR ReadyMix(TM); ROX(TM); with ROXafor ABI instruments

Inhibitor Resistant Genotyping PCR ReadyMix(TM); ROX(TM); with ROXafor ABI instruments

Stock Code: 3582418
Manufacturer Part No: PIR02-5000RXN
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Components


2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume


Features and Benefits


Assay results in as little as 33 minutes Highly efficient and sensitive real-time PCR results Little/no optimization required


General description


Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use PCR master mix used for amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.


Legal Information


TaqMan is a registered trademark of Roche Molecular Systems, Inc.


ReadyMix is a trademark of Sigma-Aldrich Co. LLC


ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Colourlight blue
Quality Level200
ManufacturerSIGMA-ALDRICH
CompatibilityABI 7900 HT, ABI 7000, ABI 5700, ABI 7900 HT Fast, ABI 7900, ABI StepOnePlus, ABI 7700, ABI 7300
Featurehotstart, Difficult Templates/Specialty Enzymes PCR, dNTPs included
Formliquid

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