KiCqStart(R) Probe qPCR ReadyMix(TM); Low ROX(TM); with Low ROX for ABI and Stratagene instruments

KiCqStart(R) Probe qPCR ReadyMix(TM); Low ROX(TM); with Low ROX for ABI and Stratagene instruments

Stock Code: 3582328
Manufacturer Part No: KCQS05-1250RXN
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Application


KiCqStart® Probe qPCR ReadyMix™, Low ROX™ has been used as a component of reaction mixture for detection of genomic DNA extracted from parasites by multiplex quantitative polymerase chain reaction (qPCR) and Real-Time PCR (qPCR).Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.


Components


2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizerspackaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume


Features and Benefits


Assay results in as little as 33 minutes Highly efficient and sensitive real-time PCR results Little/no optimization required


General description


KiCqStart® Probe qPCR ReadyMix™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA?. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.


Legal Information


TaqMan is a registered trademark of Roche Molecular Systems, Inc.


ReadyMix is a trademark of Sigma-Aldrich Co. LLC


KiCqStart is a registered trademark of Qiagen Beverly Inc.


ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Colourlight blue
ManufacturerSIGMA-ALDRICH
CompatibilityQiagen Corbett Rotor-Gene Q, Strategene Mx3000P, ABI 7500, ABI ViiA 7, Agilent AriaMx, Strategene Mx3005P, Douglas Scientific IntelliQube, ABI 7500 Fast,
Featurehotstart, dNTPs included
Formliquid

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