KiCqStart SYBR Green qPCR ReadyMix, iQ, with fluorescein for Bio-Rad systems

KiCqStart SYBR Green qPCR ReadyMix, iQ, with fluorescein for Bio-Rad systems

Stock Code: 3582325
Manufacturer Part No: KCQS03-250RXN
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Application


KiCqStart® SYBR® Green qPCR ReadyMix™ has been used for the qPCR analysis of 16S rRNA.Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.


PCR applications: Gene expression DNA quantification CHiP


Components


2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, 20 nM fluorescein, and stabilizerspackaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume


Features and Benefits


Assay results in as little as 33 minutes Highly efficient and sensitive real-time PCR results Little/no optimization required


General description


KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.


Legal Information


SYBR is a registered trademark of Life Technologies


iQ is a trademark of Bio-Rad Laboratories, Inc.


ReadyMix is a trademark of Sigma-Aldrich Co. LLC


KiCqStart is a registered trademark of Qiagen Beverly Inc.


Other Notes


Storage Conditions:KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.

Colourcolorless
ManufacturerSIGMA-ALDRICH
Compatibilityfor use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5
Featurehotstart, dNTPs included
Formliquid

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