Meridian Bioscience Uracil DNA Glycosylase - 500 Units

Meridian Bioscience Uracil DNA Glycosylase - 500 Units

Stock Code: 2801087
Manufacturer Part No: BIO27044
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Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM). Meridian Bioscience UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Applications

  • UDG treatment of uracil-containing DNA to prevent amplification by DNA polymerases
  • Used to investigate features of protein – DNA interactions
  • Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli
  • Components: Uracil DNA Glycosylase, 10x Reaction Buffer
  • Unit Definition: One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA
  • Concentration: 1u/µl
  • Storage Conditions Uracil DNA Glycosylase can be stored for 6 months at –20°C

ManufacturerMERIDIAN BIOSCIENCE

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