Titan One Tube System

Titan One Tube System

Stock Code: 2795737
Manufacturer Part No: 11855476001
Order Now for 21 day delivery
£835.71 (exc VAT) per PK100
Quantity: - +

Application


Titan™ One Tube RT-PCR System has been used:to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNAfor determining the presence or absence of RNA templates in cloning of rare messages without the need to construct cDNA libraries for quantifying the levels of gene expression


Features and Benefits


Titan™ One Tube RT-PCR System: High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR) offers a threefold higher fidelity in comparison to Taq DNA polymerase delivers increased sensitivity due to the high efficiency of all three enzymes faster than conventional two-step reverse transcriptase (RT)-PCR allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System


General description


The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System: Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase. Delivers increased sensitivity due to the high efficiency of all three enzymes. Is faster than conventional two-step RT-PCR.


Legal Information


Titan is a trademark of Roche


Expand is a trademark of Roche


Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.﹤/ br>NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only

Quality Level100
Technique(s)RT-PCR: suitable
Parameter42-60 °C optimum reaction temp.
Featurehotstart: no, dNTPs included: no
Storage Temp.−20°C

There are no downloads for this product.